24A Soft X-ray Tomography

          

                 

                  

   Drawing  of SXT endstation, correlation with fluorescence SIM

Endstations

Soft X-ray tomography is dedicated to image frozen-hydrated bioloical specimen. The endstation integrates an on-line fluorescence structured illumination microscopy (SIM) and soft X-ray tomography (SXT) to provide biological specimen with 3D functional and structural information. The spatial resolution of SXT is 15∼30 nm for 2D imaging and 50 nm for 3D tomography, which can fill the gap between fluorescence microscopy and electron microscopy in cellular studies. Because of the high penetration of soft X-ray,  the thickness of bio-sample can be imaged directly by SXT to about 8 μm at 520 eV and to 30 μm at 2,600 eV without the need of staining and sectioning.

      

             Photography of SXT endstation

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Techniques

  • Soft X-ray Tomography (SXT)
  • Fluorescence Structured-Illumination Microscopy (SIM)

Disciplines

  • Medicine and drug discovery
  • X-ray optics
  • Biomedical Engineering
  • Soft X-ray imaging
  • Bio-medical imaging

General Information

  • Source: 24A
  • Energy Range: 260-2,600 eV
  • Focused Spot Size: 20 ~ 30 nm in 2D imaging; 50 nm in 3D imaging
  • Status: under construction







National Synchrotron Radiation Research Center

101 Hsin-Ann Road, Hsinchu Science Park, Hsinchu, Taiwan 30076

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