24A Soft X-ray Tomography

Endstations Description

Soft X-ray tomography is dedicated to image frozen-hydrated bioloical specimen. The endstation integrates an on-line fluorescence structured illumination microscopy (SIM) and soft X-ray tomography (SXT) to provide biological specimen with 3D functional and structural information. The spatial resolution of SXT is 15∼30 nm for 2D imaging and 50 nm for 3D tomography, which can fill the gap between fluorescence microscopy and electron microscopy in cellular studies. Because of the high penetration of soft X-ray,  the thickness of bio-sample can be imaged directly by SXT to about 8 μm at 520 eV and to 30 μm at 2,600 eV without the need of staining and sectioning.



  1. SIM is not open to user yet.
  2. Endstation is only open to use material samples or BSL-1 bioloical specimens.

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  • Soft X-ray Tomography (SXT)
  • Fluorescence Structured-Illumination Microscopy (SIM)


  • Medicine and drug discovery
  • X-ray optics
  • Biomedical Engineering
  • Soft X-ray imaging
  • Bio-medical imaging

General Information

  • Source: 24A
  • Energy Range: 260-2,600 eV
  • Focused Spot Size: 20 ~ 30 nm in 2D imaging; 50 nm in 3D imaging
  • Status: under construction

National Synchrotron Radiation Research Center

101 Hsin-Ann Road, Hsinchu Science Park, Hsinchu, Taiwan 30076