05A Protein Microcrystallography

Scientific Scope

3D biostructures of:

  • Viruses and large macromolecular assemblies
  • Multi-protein and tertiary complexes
  • Membrane proteins
  • Pathogenicity-related proteins
  • Extreme-environment metalloproteins
  • Structure-based drug designs
  • Structural genomics projects

Protein crystals



Protein and virus structures

Beamline Description

The routine use of synchrotron radiation for single crystal diffraction study in the past decades has revolutionized macromolecular structural biology. However, crystals of important macromolecules, such as membrane proteins and viruses, are usually small in sizes and have poor diffraction quality. Advances in synchrotron radiation sources, detectors, and software are necessary to tackle these challenging problems. TPS 05A aims for difficult structures as well as routine data collection. The X-ray source of TPS 05A is a three meters long in-vacuum undulator (IU22), producing a high-brilliant X-ray beam. The X-rays are monochromated by a liquid-nitrogen-cooling Si double-crystal monochromator, and focused by a pair of Kirkpatrick–Baez mirrors. The focused beam size at the sample is 65 μm (H) x 36 μm (V) with photon flux of 1.1 x1013 photons/s at 12.4keV. Apertures are used to collimate the beam in the range of 50–5 μm. The beam divergence at the sample is less than 500 μrad (H) and 100 μrad (V), and the energy range is from 5.7 to 20 keV (wavelength 2.175-0.62 Å). Equipped with a high speed CCD area detector and a micro-diffractometer, TPS 05A is capable of not only shutterless data collection for much higher throughput and helical scan to mitigate the radiation damage but also grid scan to find the best diffraction areas or to locate micro crystals. The optional mini-κ goniometer of the high precision micro-diffractometer enables crystal reorientation. A robotic sample changer for automatically sample mounting and centering in installed, making the data acquisition more efficient. Substantial user-support, remote access and mail-in service are also provided.

Summary of beam properties

Beam properties of PX beamlines at the NSRRC

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 Contacts

Endstation

—+886-3-578-0281 Ext. 2051

BL Spokesperson

—Yuch-Cheng Jean (簡玉成)
ycjean@nsrrc.org.tw
+886-3-578-0281 Ext. 7321

BL Manager/Local Contact

Chien-Chang Tseng (曾建璋)
marksman@nsrrc.org.tw
+886-3-578-0281 Ext. 7310

BL Local Contact

Chung-Kuang Chou (周重光)
ck.chou@nsrrc.org.tw
+886-3-578-0281 Ext. 7310


Techniques

  • Large unit-cell crystallography
  • Microcrystallography
  • High throughput crystallography
  • 0.7–2 Å MAD/SAD phasing
  • Protein crystallography
  • Macromolecular crystallography
  • Biological crystallography
  • Protein microcrystallography

Disciplines

  • Structural biology
  • Biochemistry
  • Structural genomics
  • Medicine and drug discovery

General Information

  • Source: 05A
  • Energy Range: 5.7–20 keV
  • Focused Spot Size: 65 x 36 μm² (H x V)
  • Status: operational







National Synchrotron Radiation Research Center

101 Hsin-Ann Road, Hsinchu Science Park, Hsinchu, Taiwan 30076

+886-3-578-0281