24A Soft X-ray Tomography
24A is a full-field transmission soft X-ray tomography beamline. Microscopy includes an on-line correlation of fluorescence structured-illumination microscopy (SIM) with soft X-ray tomography (SXT), which is dedicated to image frozen-hydrated biological specimen with a spatial resolution of 15-30 nm for 2D imaging and 50 nm for 3D tomography.
Unique Features
To image 3D cellular strustures by the correlation of fluorescence structured-illumination microscopy with soft X-ray tomography.
Scientific Opportunities
• Molecular events within cells: aggregation of misfolded proteins in cells
• Sub-cellular organelles: endocytosis, exocytosis, cell migration and cellular changes upon stimulation
• Cell-cell interaction and communication: neurological synapse and immunological synapse
• Host-microbe interaction: virus and intracellular bacteria
Beamline Specifications
• Energy range: 260∼2600 eV
• Monochromator: varied-line-space plane grating of 600 l/mm ruling density
• Resolving power: 2,000
• Flux @ 520 eV: 2.8 x 1011 phs/s with 0.05% BW at virtual source
Beamline optical layout
Station features
• Correlation of SIM with SXT
• Tomography: cryo-condition
• Detector: Andor iKon-L Series imaging CCD
Endstation
Sample environment
• High vacuum condition
• Liquid N2 cryogenics
• Cryo-sample load-lock system
• Bio-lab with biosafety level 2
• Low humidity lab for cryo-sample preparation