24A Soft X-ray Tomography

24A is a full-field transmission soft X-ray tomography beamline. Microscopy includes an on-line correlation of fluorescence structured-illumination microscopy (SIM) with soft X-ray tomography (SXT), which is dedicated to image frozen-hydrated biological specimen with a spatial resolution of 15-30 nm for 2D imaging and 50 nm for 3D tomography.

Unique Features
To image 3D cellular strustures by the correlation of fluorescence structured-illumination microscopy with soft X-ray tomography.

Scientific Opportunities
•  Molecular events within cells: aggregation of misfolded proteins in cells

•  Sub-cellular organelles: endocytosis, exocytosis, cell migration and cellular changes upon stimulation

•  Cell-cell interaction and communication: neurological synapse and immunological synapse

•  Host-microbe interaction: virus and intracellular bacteria

Beamline Specifications

•  Energy range: 260∼2600 eV

•  Monochromator: varied-line-space plane grating of 600 l/mm ruling density

•  Resolving power: 2,000

•  Flux @ 520 eV: 2.8 x 1011 phs/s with 0.05% BW at virtual source

         
                                        Beamline optical layout


Station features

•  Correlation of SIM with SXT

•  Tomography: cryo-condition

•  Detector: Andor iKon-L Series imaging CCD

               

                                           Endstation

Sample environment

•  High vacuum condition

•  Liquid N2 cryogenics

•  Cryo-sample load-lock system

•  Bio-lab with biosafety level 2

•  Low humidity lab for cryo-sample preparation

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Techniques

  • Soft X-ray Tomography (SXT)
  • Fluorescence Structured-Illumination Microscopy (SIM)

Disciplines

  • Medicine and drug discovery
  • X-ray optics
  • Biomedical Engineering
  • Soft X-ray imaging
  • Bio-medical imaging

General Information

  • Source: 24A
  • Energy Range: 260-2,600 eV
  • Focused Spot Size: 20 ~ 30 nm in 2D imaging; 50 nm in 3D imaging
  • Status: under construction







National Synchrotron Radiation Research Center

101 Hsin-Ann Road, Hsinchu Science Park, Hsinchu, Taiwan 30076

+886-3-578-0281