24A is a full-field transmission soft X-ray tomography beamline. Microscopy includes an on-line correlation of fluorescence structured-illumination microscopy (SIM) with soft X-ray tomography (SXT), which is dedicated to image frozen-hydrated biological specimen with a spatial resolution of 15-30 nm for 2D imaging and 50 nm for 3D tomography.

To image 3D cellular strustures by the correlation of fluorescence structured-illumination microscopy with soft X-ray tomography.

• Molecular events within cells: aggregation of misfolded proteins in cells.
• Sub-cellular organelles: endocytosis, exocytosis, cell migration and cellular changes upon stimulation.
• Cell-cell interaction and communication: neurological synapse and immunological synapse.
• Host-microbe interaction: virus and intracellular bacteria.

Endstation

—+886-3-578-0281 Ext. 2242

BL Spokesperson

Lai, Lee-Jene (賴麗珍)
jene@nsrrc.org.tw
+886-3-578-0281 Ext. 7318

BL Manager/Local Contact

Lin, Zi-Jing (林子敬)
lin.zj@nsrrc.org.tw
+886-3-578-0281 Ext. 7204

• Soft X-ray Tomography (SXT)
• Fluorescence Structured-Illumination Microscopy (SIM)

• Medicine and drug discovery
• X-ray optics
• Biomedical Engineering
• Soft X-ray imaging
• Bio-medical imaging

• Source: Bending Magnet
• Energy Range: 260-2,600 eV
• Focused Spot Size: 20 ~ 30 nm in 2D imaging; 50 nm in 3D imaging
• Status: under construction